Lazio came out with more purpose in the early moments of the second period, and Sissoko's name was taken by referee Saccani after he brought down Kolarov in the middle of a fine solo run through the centre.
But it wasn't long before Juve were back on the attack, and Antonio Candreva unleashed a swinging 20-yard effort towards Muslera's bottom corner, which the Uruguayan did well to get down to and make the save.
The home side then produced the best moment of the match, and it was a piece of individual magic from Diego. With seemingly nothing on 20 yards from goal, the Brazilian fired in a left foot shot with little backlift which cannoned off the outside of Muslera's near post, leaving Lazio to breathe a huge sigh of relief.
The Bianconeri soon managed to build up a spell of pressure, and Paolo De Ceglie stood up an excellent cross for the head of Sissoko eight yards from goal, but the Malian couldn't direct his effort on target under pressure.
The breakthrough came in the 69th minute when Candreva chipped in a clever cross, Amauri flicked on and Del Piero turned superbly. But as the skipper shaped up to shoot left-footed he was brushed by Diakite, and the referee saw the contact fit enough to reward the Bianconeri a penalty. Del Piero picked himself up and fired low to Muslera's left, sending the keeper the wrong way and giving Juventus the lead.
As the two coaches looked to make changes and the pace seemingly gone from the game, proceedings suddenly came alive once more as Lazio scored a shock equaliser.
Roberto Baronio's right wing free-kick was half cleared by Amauri, but Zarate was able to chip the ball back into the box, and Mauri beat Zdenek Grygera to the ball on the slide and managed to poke the ball past Alex Manninger's dive.
Mauri picked up a head wound in celebrating his goal and was replaced by Sebastiano Siviglia as a result, with coach Davide Ballardini seemingly bracing his side for an Alamo-style attack from the home side.
The siege came thick and fast from the home side, but they just couldn't force a clear goalscoring opportunity, and their efforts became more and more frantic as time ran out.
Michele Paolucci was introduced by Zaccheroni in place of Del Piero late on, but he was unable to help his new boss picked up the much sought after three points.